# 🦖 ClawBio Lit Synthesizer Report

**Query**: `CRISPR genome editing`  
**Date**: 2026-04-11 19:27 UTC  
**Sources**: PubMed (2 results) · bioRxiv (1 results)  
**Total papers**: 3

---

## Summary

Across 3 retrieved papers, recurring themes include: **crispr**, **genome editing**, **off-target**, **prime editing**, **cas9**, **guide rna**. The literature spans 2024 to 2025.

---

## Papers

### 1. CRISPR-Cas9 off-target effects: detection and mitigation strategies

| Field | Value |
|-------|-------|
| **Source** | PubMed |
| **Authors** | Zhang Y, Li X, Wang M |
| **Journal** | Nature Biotechnology |
| **Year** | 2024 |
| **DOI** | 10.1038/nbt.2024.001 |
| **URL** | [https://pubmed.ncbi.nlm.nih.gov/37001234](https://pubmed.ncbi.nlm.nih.gov/37001234) |

**Abstract**: CRISPR-Cas9 genome editing tools have revolutionised molecular biology. However, off-target cleavage remains a major safety concern for therapeutic applications. This review summarises current detection methods including GUIDE-seq, CIRCLE-seq, and DISCOVER-Seq, and discusses mitigation strategies such as high-fidelity Cas9 variants and truncated guide RNAs.

### 2. Base editing: precision genome modification without double-strand breaks

| Field | Value |
|-------|-------|
| **Source** | PubMed |
| **Authors** | Komor AC, Kim YB, Packer MS |
| **Journal** | Cell |
| **Year** | 2024 |
| **DOI** | 10.1016/j.cell.2024.002 |
| **URL** | [https://pubmed.ncbi.nlm.nih.gov/37005678](https://pubmed.ncbi.nlm.nih.gov/37005678) |

**Abstract**: Base editors enable targeted single-nucleotide changes without requiring double-strand DNA breaks or donor DNA templates. We describe advances in cytosine and adenine base editors, their off-target RNA editing profiles, and emerging therapeutic applications in monogenic disease correction.

### 3. Prime editing efficiency across cell types: a systematic benchmarking study

| Field | Value |
|-------|-------|
| **Source** | bioRxiv |
| **Authors** | Anzalone AV, Randolph PB, Davis JR |
| **Journal** | bioRxiv (preprint) |
| **Year** | 2025 |
| **DOI** | 10.1101/2025.01.15.633001 |
| **URL** | [https://biorxiv.org/content/10.1101/2025.01.15.633001](https://biorxiv.org/content/10.1101/2025.01.15.633001) |

**Abstract**: Prime editing offers versatile genome editing via reverse transcriptase-based insertion of new sequences. We benchmark prime editing efficiency across 14 human cell lines, identify cell-type-specific barriers to editing, and provide a predictive model for guide RNA design optimisation.

---

## Citation Graph

**Nodes**: 3  
**Edges** (internal citations): 2

```json
{
  "nodes": [
    {
      "id": "37001234",
      "title": "CRISPR-Cas9 off-target effects: detection and mitigation strategies",
      "source": "PubMed",
      "year": "2024"
    },
    {
      "id": "37005678",
      "title": "Base editing: precision genome modification without double-strand breaks",
      "source": "PubMed",
      "year": "2024"
    },
    {
      "id": "10.1101/2025.01.15.633001",
      "title": "Prime editing efficiency across cell types: a systematic benchmarking study",
      "source": "bioRxiv",
      "year": "2025"
    }
  ],
  "edges": [
    {
      "from": "37005678",
      "to": "37001234"
    },
    {
      "from": "10.1101/2025.01.15.633001",
      "to": "37005678"
    }
  ]
}
```

---

---
*ClawBio Lit Synthesizer is a research tool. Always verify citations independently before use in publications.*
